Objectives:
- Match restriction enzyme names to the bacteria in which they are naturally found.
- Describe the role of restriction enzymes in nature and in biotechnology applications.
- Contrast sticky vs. blunt ends.
- Identify a restriction site as either leaving sticky or blunt ends.
- Demonstrate accurate use of a micro-pipette.
- Determine the number and size of DNA fragments after being exposed to restriction enzymes (both linear and plasmid DNA).
- Explain the function and purpose of DNA electrophoresis.
- Describe how and why DNA fragments separate during electrophoresis.
- Outline the functions of the buffer, marker and loading dye in DNA electrophoresis.